Why mitochondrial function critical for growing on pyruvate | Homework Helpers

Q. You find out a mutant yeast strain that cannot grow while provided with pyruvate as its sole carbohydrate, though it grows normally when given glucose as a carbon energy source; you call this the Pyr- trait. Wild-type yeast can grow well using either carbon source; you designate this Pyr+ trait. You suppose that the mutant yeasts have defective mitochondria.

A. Why is mitochondrial function critical for growing on pyruvate?

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B. You want to find out whether the mutation responsible for the Pyr- trait is carried in the nuclear or mitochondrial genome. Your experiment is to mate Pyr- haploid cells to Pyr+ haploid cells and examine segregation of the trait in the progeny. After mating, the hybrid diploid cells can grow on pyruvate (Pyr+). You send these diploid cells through meiosis and examine the four haploid cells generated from a single diploid cell. If the mutation is in mitochrondrial DNA, will the four haploid cells probably be all Pyr-, all Pyr+, or a combination? What if the mutation is in nuclear DNA?

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