Solution-Describe how you would control contamination | Homework Helpers

You have been discussing your new “molecular prowess” and people have noticed. You have been given the opportunity to propose a molecular assay and develop a molecular laboratory. This will require that you write a proposal to administration. This proposal must include your assay design and equipment list. You must propose how you would use the available space given the included floor plan. Each section of this exercise will require that you adhere to the space limitations.

1. ONE PAGE MAXIMUM

Don't use plagiarized sources. Get Your Custom Essay on
Solution-Describe how you would control contamination | Homework Helpers
Get an essay WRITTEN FOR YOU, Plagiarism free, and by an EXPERT!
Order Essay

The gene you will need to design a test around is provided. The polymorphism is noted:

5′
301 gtcagcccca tggtggtggc tggggacagc cacatggtgg tggaggctgg ggtcaaggtg

361 gtagccacag tcagtggaac aagcccagta agccaaaaac caacaygaag catgtggcag

421 gagctgctgc agctggagca gtggtagggg gccttggtgg ctacatgctg ggaagtgcca

3′
481 tgagcaggcc tcttatacat tttggcaatg actatgagga ccgttactat cgtgaaaaca

Describe in detail the assay you will design. Be as specific as possible. No make believe enzymes or reagents. Nothing magical.

At a minimum your description should include:

-A diagram indicating where any primers or probes would anneal to the above sequence (you can annotate the sequence above).
-A list of any reagents needed, i.e. oligonucleotides, enzymes, etc.
-A statement of how you will detect the polymorphisms
-Controls needed
-The concentrations and formulations of any gels used
-The concentrations and components of any enzymatic reactions used
-A diagram of how the results of your assay would appear including controls, a homozygous individual and a heterozygote.

Priniciple of test:

Reagents needed:

Controls needed:

Outline of assay:

Diagram of results:

2. ONE PAGE MAXIMUM

Given the following floor plan, indicate how you would assign the activities related to your assay. Be sure to describe how you would control contamination. At a minimum it is expected that you will include areas for:

Specimen processing Data Analysis
Pre-PCR
Post-PCR
Reagent preparation Storage
Darkroom

Show where refrigerators, freezers, thermocyclers, safety equipment, hoods and other equipment.

Be sure to indicate traffic flow if it is integral to your contamination control plan.

You are not allowed to aID, move or remove walls. You can aID doors if you feel it necessary 1/2″ = 5 ft

Answered:-

Verified Expert

This assignment contains two parts: The first part of the assignment includes design of a molecular assay and the second part is related to design of molecular laboratory. In the first part, design of molecular assay is done. A given DNA sequence is analysed and polymorphisms are detected from it using RFLP. In the second part, a molecular biology laboratory having 6 parts is designed.

Calculate your paper price
Pages (550 words)
Approximate price: -

Why Choose Us

Top quality papers

We always make sure that writers follow all your instructions precisely. You can choose your academic level: high school, college/university or professional, and we will assign a writer who has a respective degree.

Professional academic writers

We have hired a team of professional writers experienced in academic and business writing. Most of them are native speakers and PhD holders able to take care of any assignment you need help with.

Free revisions

If you feel that we missed something, send the order for a free revision. You will have 10 days to send the order for revision after you receive the final paper. You can either do it on your own after signing in to your personal account or by contacting our support.

On-time delivery

All papers are always delivered on time. In case we need more time to master your paper, we may contact you regarding the deadline extension. In case you cannot provide us with more time, a 100% refund is guaranteed.

Original & confidential

We use several checkers to make sure that all papers you receive are plagiarism-free. Our editors carefully go through all in-text citations. We also promise full confidentiality in all our services.

24/7 Customer Support

Our support agents are available 24 hours a day 7 days a week and committed to providing you with the best customer experience. Get in touch whenever you need any assistance.

Try it now!

Calculate the price of your order

Total price:
$0.00

How it works?

Follow these simple steps to get your paper done

Place your order

Fill in the order form and provide all details of your assignment.

Proceed with the payment

Choose the payment system that suits you most.

Receive the final file

Once your paper is ready, we will email it to you.

Our Services

No need to work on your paper at night. Sleep tight, we will cover your back. We offer all kinds of writing services.

Essays

Essay Writing Service

You are welcome to choose your academic level and the type of your paper. Our academic experts will gladly help you with essays, case studies, research papers and other assignments.

Admissions

Admission help & business writing

You can be positive that we will be here 24/7 to help you get accepted to the Master’s program at the TOP-universities or help you get a well-paid position.

Reviews

Editing your paper

Our academic writers and editors will help you submit a well-structured and organized paper just on time. We will ensure that your final paper is of the highest quality and absolutely free of mistakes.

Reviews

Revising your paper

Our academic writers and editors will help you with unlimited number of revisions in case you need any customization of your academic papers