In a restriction mapping experiment using the plasmid | Homework Helpers

In a restriction mapping experiment using the plasmid P(ST), you cleave the circular plasmid with the restrictionenzymes XbaI, Asp718, BamHI and EcoRI individually and in variouscombinations with each other. You obtain DNA fragments with thefollowing sizes:

Enzymes Used      Size of DNA fragments(bp)
BamHI, EcoRI, XbaI       3000, 1850,1250
Asp718, BamHI,XbaI                 3000, 2500, 600
Asp718,BamHI                                     5500, 600
Asp718,XbaI                                      3600, 2500
BamHI,XbaI                                       3100, 3000
Asp                                                        718 6100
BamHI                                                           6100
XbaI                                                               6100

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Using the above data, make a map of the P(ST)plasmid indicating the distances between each restriction site foreach of the four enzymes.

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