Determine the amino acid sequence using the genetic code | Homework Helpers

As a World-famous medical doctor that dabbles in research from time to time, you’ve taken a recent interest in a newly discovered compound that appears to cure terriblitis, which has a variety of terrible symptoms.  However, this drug appears to work in only 33% of patients, while the remaining 67% seem completely immune to the drug.  You head to your basement lab with your data to solve the mystery.

1. You have complete family history for a large family in which every family member is affected by terribilitis.  You’ve constructed a family tree to discern the heritability of the drug-susceptibility phenotype. 

Don't use plagiarized sources. Get Your Custom Essay on
Determine the amino acid sequence using the genetic code | Homework Helpers
Get an essay WRITTEN FOR YOU, Plagiarism free, and by an EXPERT!
Order Essay

1346_Figure.png

a. The shaded boxes indicate individuals that were cured by the new drug.  The clear boxes indicate individuals for whom the drug had no effect.  (Dark grey boxes indicate individuals that have not been tested.)  The individual marked with the arrow is called the ‘propositus’, or the individual under study. Based on this information, do you believe the father of the propositus could be curedby the drug?Why or why not?

b. Based on the father’s predicted genotype, what is the probability that the propositus could be cured with the drug? 

2. Using standard molecular techniques, you determine that the limaglobingene is responsible for drug susceptibility in this case. The sequence of the dominant and recessive alleles is shown below.  The sequence of the propositus is also shown (two alleles, as the individual is human and thus diploid).   Based on this data, will the propositus be able to be cured with the drug?

Dominant allele:   5′-AATAGACAGTACAGTAGGGACCGTGAGTTTAGTAG-3′

Recessive allele:  5′-AATAGACAGTACAGTAGGGACTGTGAGTTTAGTAG-3′

Propositus allele 1:    5′-AATAGACAGTACAGTAGGGACCGTGAGTTTAGTAG-3′

Propositus allele 2:    5′-AATAGACAGTACAGTAGGGACTGTGAGTTTAGTAG-3′

3. Based on the data given in Q2, you choose to confirm with a restriction digest using an enzyme (CutII) that cuts the recessive allele on the 5′ sideof the mutation.  The dominant allele is not cut by this enzyme.  Based on this information, draw the bands in the gel below.  Use the laIDer given to determine how far the fragments will travel on the gel.

1953_Figure1.png

4. Assuming that the DNA sequence in Q2 is in frame and shows the TEMPLATE strand. Determine the amino acid sequence using the genetic code and give a chemical explaination as to why the drug binds better to the recessive allele. (Hint:  remember that mRNA is produced 5′ to 3′, which means that the DNA is read 3′ to 5′.).

5′-AATAGACAGTACAGTAGGGACCGTGAGTTTAGTAG-3′

5. You decide to expand your study to a much wider population.  You study 1000 individuals of various genotypes to see if they are receptive to the drug. You then test their genotypes.  Is your data consistent with the hypothesis that the trait is strictly Mendellian with complete dominance? (3 points)

 

Genotype RR

Genotype Rr

Genotype rr

Receptive to the drug

206

275

53

Not receptive

90

26

350

(Note: You will need to conduct a Chi-squared test.  Complete each of the following parts to do your test).

Ho:

Ha:

Degrees of freedom:

X2 value:

 

Expectation

Observation

Not cured (RR or Rr)

 

 

Cured (rr)

 

 

Because the chi-squared value is (greater than / less than) my critical value, I will (reject / accept) the null hypothesis.

Calculate your paper price
Pages (550 words)
Approximate price: -

Why Choose Us

Top quality papers

We always make sure that writers follow all your instructions precisely. You can choose your academic level: high school, college/university or professional, and we will assign a writer who has a respective degree.

Professional academic writers

We have hired a team of professional writers experienced in academic and business writing. Most of them are native speakers and PhD holders able to take care of any assignment you need help with.

Free revisions

If you feel that we missed something, send the order for a free revision. You will have 10 days to send the order for revision after you receive the final paper. You can either do it on your own after signing in to your personal account or by contacting our support.

On-time delivery

All papers are always delivered on time. In case we need more time to master your paper, we may contact you regarding the deadline extension. In case you cannot provide us with more time, a 100% refund is guaranteed.

Original & confidential

We use several checkers to make sure that all papers you receive are plagiarism-free. Our editors carefully go through all in-text citations. We also promise full confidentiality in all our services.

24/7 Customer Support

Our support agents are available 24 hours a day 7 days a week and committed to providing you with the best customer experience. Get in touch whenever you need any assistance.

Try it now!

Calculate the price of your order

Total price:
$0.00

How it works?

Follow these simple steps to get your paper done

Place your order

Fill in the order form and provide all details of your assignment.

Proceed with the payment

Choose the payment system that suits you most.

Receive the final file

Once your paper is ready, we will email it to you.

Our Services

No need to work on your paper at night. Sleep tight, we will cover your back. We offer all kinds of writing services.

Essays

Essay Writing Service

You are welcome to choose your academic level and the type of your paper. Our academic experts will gladly help you with essays, case studies, research papers and other assignments.

Admissions

Admission help & business writing

You can be positive that we will be here 24/7 to help you get accepted to the Master’s program at the TOP-universities or help you get a well-paid position.

Reviews

Editing your paper

Our academic writers and editors will help you submit a well-structured and organized paper just on time. We will ensure that your final paper is of the highest quality and absolutely free of mistakes.

Reviews

Revising your paper

Our academic writers and editors will help you with unlimited number of revisions in case you need any customization of your academic papers