Lipid rafts are specialized regions of the exoplasmic leaflet. GPI-anchored proteins are able to diffuse from the main (non-raft) area of the exoplasmic leaflet into a lipid raft and then back out into the main exoplasmic leaflet area. Which statement below is INCORRECT about these lipid regions and GPI-membrane proteins?
A. Scanning calorimetric measurements would show lipid rafts to have a higher melting temperature (Tm) than the non-raft portion of the exoplasmic leaflet.
B. The lipid raft has a higher concentration of cholesterol than does the main exoplasmic leaflet area.
C. As an integral membrane protein, the mobility of GPI-proteins in either lipid rafts or the main exoplasmic leaflet depends on the mobility of the protein’s transmembrane domain in the bilayer.
D. FRAP measurements of a GPI-anchored protein would show higher mobility (faster recovery) rates when the protein’s lipid anchor is moving through the main area of the exoplasmic leaflet compared to its mobility within a lipid raft.
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