Solution-Expression during sex determination in drosophila | Homework Helpers

To investigate transformer (tra) gene expression during sex determination in Drosophila, you perform in situ hybridization (FISH) and immunostaining. Fill in the table with the results you expect to observe following the example given in the first row.

Experiment

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Female embryos

Male embryos

Immunostaining with an antibody to Sxl protein

stain positively

no staining

FISH with a probe to exon 2 of tra

FISH with a probe to exon 3 of tra

Immunostaining with an antibody to Tra protein

Match sex phenotype to each genotype of a transformer mutant fly:

XX: ___________________________;     XY: ________________________

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