The fluorescence of each of a series of acidic solutions of quinine was determined five times. The results are given below.
| Concentration, ng ml-1 Don't use plagiarized sources. Get Your Custom Essay on Solution-Concentrations of solutions with fluorescence | Homework Helpers Get an essay WRITTEN FOR YOU, Plagiarism free, and by an EXPERT! | 0 | 10 | 20 | 30 | 40 | 50 |
| Fluorescence intensity | 4 | 22 | 44 | 60 | 75 | 104 |
| (arbitrary units) | 3 | 20 | 46 | 63 | 81 | 109 |
| 4 | 21 | 45 | 60 | 79 | 107 | |
| 5 | 22 | 44 | 63 | 78 | 101 | |
| 4 | 21 | 44 | 63 | 77 | 105 |
Determine the slopes and intercepts of the un-weighted and weighted regression lines. Calculate, using both regression lines, the confidence limits for the concentrations of solutions with fluorescence intensities of 15 and 90 units. Compare the results of the unweighted regression to those of weighted regression.
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